Plasminogen Activator Inhibitor (PAI 1) 4G/5G

The human Plasminogen Activator Inhibitor-1 (PAI-1) is one of the strongest inhibitors of fibrinolytic activities. Due to an induction of the transcription, the homocygot 4G/4G genotype is associated with higher levels of PAI-1 and cholesterol in plasma. This finding may explain the involvement of the PAI-1 4G/5G polymorphism in the clustering of atherothrombotic risk factors, and why people with the 4G/4G genotype are at increased risk for myocardial infarction.

Kit with reagents for the detection of the human PAI 4G/5G polymorphism using the LightCycler^® 1.x / 2.0 / 480 Instruments.

Description

A 345 bp fragment of the human PAI-1 gene is amplified with specific primers. The resulting PCR fragments are analyzed with hybridization probes labeled with LightCycler^® Red 640 (detected in channel 640) The genotype is identified by running a melting curve with specific melting points (Tm). The human PAI-1 4G DNA exhibits a Tm of 54.5°C in channel 640 and the human PAI-1 5G DNA exhibits a Tm of 62.0°C in the same channel.

The supplied control DNA allows for the accurate comparison with unknown samples.

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