Ovine PRNP Variants

Scrapie is a fatal neurodegenerative disease of sheep that belongs to the group of prion diseases. The bovine spongiform encephalopathy (BSE) and the new Creutzfeldt-Jakob (nCJD) disease in humans are closely related. The host encoded prion protein (PRNP) plays a central role in the disease process.

PRNP gene polymorphisms have been found to be associated with disease susceptibility and pathogenesis. Based on the triplet sequences present at codons 136, 154 and 171, the most important allelic variants reported are PRNP VRQ, ARR, ARQ, ARH and AHQ.

The LightMix^® Kit allows the detection of the variant 136 Val, 154 His and 171 Qln and Arg with the LightCycler^® 1.x and 2.0 instruments. For the LightCycler^® 480 (and 2.0) instruments we offer an alternative kit 40-0297-96 which detect also the Nor98 (atypical scrapie) variant 141 Phe.

Identification of five allelic variants of the sheep PrP gene and their association with natural scrapie. Belt PB, Muileman IH, Schreuder BE, Bos-de Ruijter J, Gielkens AL, Smits MA. J Gen Virol. 1995 Mar;76 ( Pt 3):509-17.

Additional reagents and items required

High Pure PCR Template preparation kit	11 796 828 001
LightCycler® FastStart DNA Master HybProbe	03 003 248 001
Capillaries (20 µl) (LC1.x / 2.0 Instruments)	04 929 292 001

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